RNA Barcoding

Betainfluenzavirus

Influenza B virus

Sequence Information

Serial numbers	Length	P value	Max total score	Max E value	BSW score	Regions
1	35	0.0045	65.8	9.00E-08	4.17	PB2
>Barcode_1 AGAATATCAGGAAGAGGATTCAAAAATGATGAAGA Forward primer AGAATATCAGGAAGAGGAT Reverse primer TCTTCATCATTTTTGAATC
2	35	0.0045	65.8	9.00E-08	4.17	PB2
>Barcode_2 GACATGTTCCAATGGGATGCATTTGAAGCATTTGA Forward primer GACATGTTCCAATGGGATG Reverse primer TCAAATGCTTCAAATGCA
3	34	0.0052	63.9	3.00E-07	4.09	PB2
>Barcode_3 TAACTATATGCGGCAGAATGATGTCATTAAAAGG Forward primer TAACTATATGCGGCAGAA Reverse primer CCTTTTAATGACATCATTC
4	31	0.0083	58.4	1.00E-05	3.85	PB1
>Barcode_4 CAGTAAAGAATATAAAGAAAAAATTGCCTGC Forward primer CAGTAAAGAATATAAAGAA Reverse primer GCAGGCAATTTTT
5	32	0.0071	60.2	3.00E-06	3.93	PB1
>Barcode_5 TTTGTATTAGTAGTTGAAAACTTGGCTAAAAA Forward primer TTTGTATTAGTAGTTGAAA Reverse primer TTTTTAGCCAAGTTTTCAA
6	32	0.0071	60.2	3.00E-06	3.93	PB1
>Barcode_6 ATGATGATGGGAATGTTTAATATGCTATCTAC Forward primer ATGATGATGGGAATGTTT Reverse primer GTAGATAGCATATTAAACA
7	40	0.0021	75.0	3.00E-10	4.55	PB1
>Barcode_7 TATTGGGAATAAACATGAGCAAAAAGAAAAGTTACTGTAA Forward primer TATTGGGAATAAACATGAG Reverse primer TTACAGTAACTTTTCTTTT
8	65	0.0001	121	2.00E-23	6.08	PA
>Barcode_8 TTCCAGACTACAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGAATT Forward primer TTCCAGACTACAATAATAC Reverse primer AATTCAGGATCTTCACT
9	35	0.0045	65.8	9.00E-08	4.17	PA
>Barcode_9 GGATTGGCTGATGATTACTTTTGGAAAAAGAAAGA Forward primer GGATTGGCTGATGATTACT Reverse primer TCTTTCTTTTTCCAAAAGT
10	37	0.0033	69.4	1.00E-08	4.32	PA
>Barcode_10 ACCATGAGCTACCAGAAGTTCCATATAATGCCTTTCT Forward primer ACCATGAGCTACCAGAAGT Reverse primer AGAAAGGCATTATATGGAA
11	35	0.0045	65.8	9.00E-08	4.17	PA
>Barcode_11 AGAGTGGACTCAGGAAAGTGGCCAAAATATACTGT Forward primer AGAGTGGACTCAGGAAAGT Reverse primer ACAGTATATTTTGGCCACT
12	36	0.0038	67.6	3.00E-08	4.25	HA
>Barcode_12 AAAATGAAGGCAATAATTGTACTACTCATGGTAGTA Forward primer AAAATGAAGGCAATAATTG Reverse primer TACTACCATGAGTA
13	44	0.0011	82.4	3.00E-12	4.87	HA
>Barcode_13 GGAGGATGGGAAGGAATGATTGCAGGTTGGCACGGATACACATC Forward primer GGAGGATGGGAAGGAATGA Reverse primer GATGTGTATCCGTGCCAAC
14	37	0.0033	69.4	1.00E-08	4.32	NP
>Barcode_14 TAAAAGAACTGAAAATCAAAATGTCCAACATGGATAT Forward primer TAAAAGAACTGAAAATCAA Reverse primer ATATCCATGTTGG
15	39	0.0024	73.1	1.00E-09	4.48	NP
>Barcode_15 AATTACCTTTTTAAAAGAAGAGGTGAAAACAATGTACAA Forward primer AATTACCTTT Reverse primer TTGTACATTGTTTTCACCT
16	44	0.0011	82.4	3.00E-12	4.87	NP
>Barcode_16 AGACCTATTGCTCTAAGCAAGCAAGCTGTAAGAAGAATGCTGTC Forward primer AGACCTATTGCTCTAAGC Reverse primer GACAGCATTCTTCTTACAG
17	32	0.0071	60.2	3.00E-06	3.93	NP
>Barcode_17 CGTGATGCAGATGTCAAAGGAAATCTACTCAA Forward primer CGTGATGCAGATGTCAAAG Reverse primer TTGAGTAGATTTCCTTTGA
18	63	0.0001	117	2.00E-22	6.07	NP
>Barcode_18 AAGAAAACCAGTGGAAATGCTTTCATTGGGAAGAAAATGTTTCAAATATCAGACAAAAACAAA Forward primer AAGAAAACCAGTGGAAATG Reverse primer TTTGTTTTTGTCTGATATT
19	79	0.0001	147	4.00E-31	6.17	NP
>Barcode_19 ATTCCAATTAAGCAGACCATCCCCAATTTCTTCTTTGGGAGGGACACAGCAGAGGATTATGATGACCTCGATTATTAAA Forward primer ATTCCAATTAAGCAGACCA Reverse primer TTTAATAATCGAGGTCATC
20	32	0.0083	60.2	3.00E-06	3.86	NA
>Barcode_20 AGGCCAAAAAATGAACAATGCTACCTTCAACT Forward primer AGGCCAAAAA Reverse primer AGTTGAAGGTAGCATTGTT
21	31	0.0083	58.4	1.00E-05	3.85	NA
>Barcode_21 TAAAATATGGAGAAGCATATACTGACACATA Forward primer TAAAATATGGAGAAGCAT Reverse primer TATGTGTCAGTATATGCTT
22	31	0.0083	58.4	1.00E-05	3.85	M
>Barcode_22 AATGTCGCTGTTTGGAGACACAATTGCCTAC Forward primer AATGTCGCTGTTTGGAGAC Reverse primer GTAGGCAATTGTGTCTCCA
23	30	0.0097	60.0	1.00E-05	3.79	M
>Barcode_23 AGAAGATGGAGAAGGCAAAGCAGAACTAGC Forward primer AGAAGATGGAGAAGGCAAA Reverse primer GCTAGTTCTGCTTTGCCT
24	35	0.0045	65.8	9.00E-08	4.17	M
>Barcode_24 TTATCAGCTCTCCATTTCATGGCTTGGACAATAGG Forward primer TTATCAGCTCTCCATTTCA Reverse primer CCTATTGTCCAAGCCATGA
25	34	0.0052	63.9	3.00E-07	4.09	M
>Barcode_25 ACTGTATTTCTTACTATGCATTTAAGCAAATTGT Forward primer ACTGTATTTCTTACTATGC Reverse primer ACAATTTGCTTAAATGCA
26	44	0.0011	82.4	3.00E-12	4.87	NS
>Barcode_26 ACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCAC Forward primer ACCACAACACAAATTGAGG Reverse primer GTGGCATTGGTTG
27	33	0.0061	62.1	9.00E-07	4.01	NS
>Barcode_27 TCTGCTGGAATTGAAGGGTTTGAGCCATACTGT Forward primer TCTGCTGGAATTGAAGGGT Reverse primer ACAGTATGGCTCAAACCCT
28	63	0.0001	117	2.00E-22	6.07	NS
>Barcode_28 TGGGAGTCTTATCCCAATTTGGTCAAGAGCACCGATTATCACCAGAAGAGGGAGACAATTAGA Forward primer TGGGAGTCTTATCC Reverse primer TCTAATTGTCTCCCTCTTC
29	53	0.0003	99	5.00E-17	5.52	NS
>Barcode_29 GAAACATTGTATGAAATGAAGGATGTGGTTGAAGTGTACAGCAGGCAGTGCTT Forward primer GAAACATTGTATGAAATGA Reverse primer AAGCACTGCCTG